snai1 pgl2 (Addgene inc)
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snai1 pgl2
Snai1 Pgl2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/snai1+pgl2/Snail_pGL2+(Plasmid+%2331694)/pmc03711427__supp_gkt410_nar___02409___v___2012___File003-1-214-213
Average 92 stars, based on 20 article reviews
Snai1 Pgl2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/snai1+pgl2/Snail_pGL2+(Plasmid+%2331694)/pmc03711427__supp_gkt410_nar___02409___v___2012___File003-1-214-213
Average 92 stars, based on 20 article reviews
snai1 pgl2 - by Bioz Stars,
2026-09
92/100 stars
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Plasmid Preparation:Article Title: IGF2BP1 promotes mesenchymal cell properties and migration of tumor-derived cells by enhancing the expression of LEF1 and SNAI2 (SLUG) Article Snippet: Table T1: plasmids Table T2: siRNAs Figure S9, Zirkel et al. Table T3: antibodies Table T4: oligos plasmids vector cloning insertion via sense oligo (5' to 3') antisense oligo (5' to 3') Flag‐LEF1 pcDNA3.1‐Flag zeo RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac pLVX‐LEF1 pLVX puro‐ newMCS RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac GFP‐ZBP1 pEGFPC1 previously described (Huttelmaier et al., Nature 2005) Luc‐LEF1‐(A) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ccGAATTCaacatggtggaaaacgaagctcattcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐LEF1‐(B) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ctGAATTCaaacccagactgtctccacggcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐SNAI2‐3'UTR pmirGLO RT‐PCR from HT‐144 cells, Zero Blunt (Life Technologies) BamHI XhoI GGATCCGCTAGCgtgacgcaatcaatgtttactcgaacag TCTAGACTCGAGcttgttaacaaacaattctttgtacagtgg promotor constructs FN‐839 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGaaaaagtaaactgttactttgtcc ggAGATCTgttgagacggtgggggagag FN‐789 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGacttccccgggatctgcaaagcgcc ggAGATCTgttgagacggtgggggagag FN‐739 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGaagctcattaaaggtctctgttc ggAGATCTgttgagacggtgggggagag FN‐689 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGcctttctcagagccagacaggcac ggAGATCTgttgagacggtgggggagag FN‐559 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ggCTCGAGggcagccccgccctgggactg ggAGATCTgttgagacggtgggggagag FN+1 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ggCTCGAGgcccgcgccggctgtgctgcac ggAGATCTgttgagacggtgggggagag FN‐839∆4 pGL4.21 PCRs based on FN‐839 using FN‐839 sense and antisense oligos and following primers; three Cloning:Article Title: IGF2BP1 promotes mesenchymal cell properties and migration of tumor-derived cells by enhancing the expression of LEF1 and SNAI2 (SLUG) Article Snippet: Table T1: plasmids Table T2: siRNAs Figure S9, Zirkel et al. Table T3: antibodies Table T4: oligos plasmids vector cloning insertion via sense oligo (5' to 3') antisense oligo (5' to 3') Flag‐LEF1 pcDNA3.1‐Flag zeo RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac pLVX‐LEF1 pLVX puro‐ newMCS RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac GFP‐ZBP1 pEGFPC1 previously described (Huttelmaier et al., Nature 2005) Luc‐LEF1‐(A) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ccGAATTCaacatggtggaaaacgaagctcattcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐LEF1‐(B) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ctGAATTCaaacccagactgtctccacggcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐SNAI2‐3'UTR pmirGLO RT‐PCR from HT‐144 cells, Zero Blunt (Life Technologies) BamHI XhoI GGATCCGCTAGCgtgacgcaatcaatgtttactcgaacag TCTAGACTCGAGcttgttaacaaacaattctttgtacagtgg promotor constructs FN‐839 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGaaaaagtaaactgttactttgtcc ggAGATCTgttgagacggtgggggagag FN‐789 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGacttccccgggatctgcaaagcgcc ggAGATCTgttgagacggtgggggagag FN‐739 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGaagctcattaaaggtctctgttc ggAGATCTgttgagacggtgggggagag FN‐689 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGcctttctcagagccagacaggcac ggAGATCTgttgagacggtgggggagag FN‐559 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ggCTCGAGggcagccccgccctgggactg ggAGATCTgttgagacggtgggggagag FN+1 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ggCTCGAGgcccgcgccggctgtgctgcac ggAGATCTgttgagacggtgggggagag FN‐839∆4 pGL4.21 PCRs based on FN‐839 using FN‐839 sense and antisense oligos and following primers; three Construct:Article Title: IGF2BP1 promotes mesenchymal cell properties and migration of tumor-derived cells by enhancing the expression of LEF1 and SNAI2 (SLUG) Article Snippet: Table T1: plasmids Table T2: siRNAs Figure S9, Zirkel et al. Table T3: antibodies Table T4: oligos plasmids vector cloning insertion via sense oligo (5' to 3') antisense oligo (5' to 3') Flag‐LEF1 pcDNA3.1‐Flag zeo RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac pLVX‐LEF1 pLVX puro‐ newMCS RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac GFP‐ZBP1 pEGFPC1 previously described (Huttelmaier et al., Nature 2005) Luc‐LEF1‐(A) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ccGAATTCaacatggtggaaaacgaagctcattcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐LEF1‐(B) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ctGAATTCaaacccagactgtctccacggcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐SNAI2‐3'UTR pmirGLO RT‐PCR from HT‐144 cells, Zero Blunt (Life Technologies) BamHI XhoI GGATCCGCTAGCgtgacgcaatcaatgtttactcgaacag TCTAGACTCGAGcttgttaacaaacaattctttgtacagtgg promotor constructs FN‐839 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGaaaaagtaaactgttactttgtcc ggAGATCTgttgagacggtgggggagag FN‐789 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGacttccccgggatctgcaaagcgcc ggAGATCTgttgagacggtgggggagag FN‐739 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGaagctcattaaaggtctctgttc ggAGATCTgttgagacggtgggggagag FN‐689 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGcctttctcagagccagacaggcac ggAGATCTgttgagacggtgggggagag FN‐559 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ggCTCGAGggcagccccgccctgggactg ggAGATCTgttgagacggtgggggagag FN+1 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ggCTCGAGgcccgcgccggctgtgctgcac ggAGATCTgttgagacggtgggggagag FN‐839∆4 pGL4.21 PCRs based on FN‐839 using FN‐839 sense and antisense oligos and following primers; three Polymerase Chain Reaction:Article Title: IGF2BP1 promotes mesenchymal cell properties and migration of tumor-derived cells by enhancing the expression of LEF1 and SNAI2 (SLUG) Article Snippet: Table T1: plasmids Table T2: siRNAs Figure S9, Zirkel et al. Table T3: antibodies Table T4: oligos plasmids vector cloning insertion via sense oligo (5' to 3') antisense oligo (5' to 3') Flag‐LEF1 pcDNA3.1‐Flag zeo RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac pLVX‐LEF1 pLVX puro‐ newMCS RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac GFP‐ZBP1 pEGFPC1 previously described (Huttelmaier et al., Nature 2005) Luc‐LEF1‐(A) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ccGAATTCaacatggtggaaaacgaagctcattcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐LEF1‐(B) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ctGAATTCaaacccagactgtctccacggcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐SNAI2‐3'UTR pmirGLO RT‐PCR from HT‐144 cells, Zero Blunt (Life Technologies) BamHI XhoI GGATCCGCTAGCgtgacgcaatcaatgtttactcgaacag TCTAGACTCGAGcttgttaacaaacaattctttgtacagtgg promotor constructs FN‐839 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGaaaaagtaaactgttactttgtcc ggAGATCTgttgagacggtgggggagag FN‐789 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGacttccccgggatctgcaaagcgcc ggAGATCTgttgagacggtgggggagag FN‐739 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGaagctcattaaaggtctctgttc ggAGATCTgttgagacggtgggggagag FN‐689 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGcctttctcagagccagacaggcac ggAGATCTgttgagacggtgggggagag FN‐559 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ggCTCGAGggcagccccgccctgggactg ggAGATCTgttgagacggtgggggagag FN+1 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ggCTCGAGgcccgcgccggctgtgctgcac ggAGATCTgttgagacggtgggggagag FN‐839∆4 pGL4.21 PCRs based on FN‐839 using FN‐839 sense and antisense oligos and following primers; three Ligation:Article Title: IGF2BP1 promotes mesenchymal cell properties and migration of tumor-derived cells by enhancing the expression of LEF1 and SNAI2 (SLUG) Article Snippet: Table T1: plasmids Table T2: siRNAs Figure S9, Zirkel et al. Table T3: antibodies Table T4: oligos plasmids vector cloning insertion via sense oligo (5' to 3') antisense oligo (5' to 3') Flag‐LEF1 pcDNA3.1‐Flag zeo RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac pLVX‐LEF1 pLVX puro‐ newMCS RT‐PCR, Zero Blunt (Life Technologies) BamHI EcoRI ccGGATCCatgccccaactctccggaggaggtggc ccGAATTCtcagatgtaggcagctgtcattcttggac GFP‐ZBP1 pEGFPC1 previously described (Huttelmaier et al., Nature 2005) Luc‐LEF1‐(A) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ccGAATTCaacatggtggaaaacgaagctcattcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐LEF1‐(B) pcDNA3.1 FFL RT‐PCR from HEK293 cells, Zero Blunt (Life Technologies) EcoRI XhoI ctGAATTCaaacccagactgtctccacggcc ccCTCGAGaaatgacaatttttaaaaatgttttattacaaagc Luc‐SNAI2‐3'UTR pmirGLO RT‐PCR from HT‐144 cells, Zero Blunt (Life Technologies) BamHI XhoI GGATCCGCTAGCgtgacgcaatcaatgtttactcgaacag TCTAGACTCGAGcttgttaacaaacaattctttgtacagtgg promotor constructs FN‐839 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGaaaaagtaaactgttactttgtcc ggAGATCTgttgagacggtgggggagag FN‐789 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ccttCTCGAGacttccccgggatctgcaaagcgcc ggAGATCTgttgagacggtgggggagag FN‐739 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGaagctcattaaaggtctctgttc ggAGATCTgttgagacggtgggggagag FN‐689 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ccttCTCGAGcctttctcagagccagacaggcac ggAGATCTgttgagacggtgggggagag FN‐559 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy (Promega) XhoI BglII ggCTCGAGggcagccccgccctgggactg ggAGATCTgttgagacggtgggggagag FN+1 pGL4.21 PCR from HEK293 genomic DNA, pGEMTeasy(Promega) XhoI BglII ggCTCGAGgcccgcgccggctgtgctgcac ggAGATCTgttgagacggtgggggagag FN‐839∆4 pGL4.21 PCRs based on FN‐839 using FN‐839 sense and antisense oligos and following primers; three |